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Catalog Number | orb1929828 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This M-CSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-257 amino acids from the Central region of human M-CSF. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Mouse, Rat |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
UniProt ID | P09603 |
MW | 60179 Da |
Tested applications | FC, IF, IHC-P, WB |
Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Antibody Type | Primary Antibody |
Clone Number | RB18764 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Macrophage colony-stimulating factor 1, CSF-1, M-C Read more... |
Note | For research use only |
NCBI | NP_000748.3, NP_757351.1, NP_757349.1, NP_757350.1 |
M-CSF Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of M-CSF Antibody (Center) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
M-CSF Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of M-CSF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of lysate from human placenta tissue lysate, using M-CSF Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.