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CSF1 Antibody (Biotin)
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF (human Granulocyte Colony Stimulating Factor). |
| Target | CSF1 |
| Purification | Anti-hM-CSF specific antibody was purified by affinity chromatography and then biotinylated. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CSF1 Rabbit Polyclonal Antibody (Biotin) [orb2119363]
IF, IHC, WB
Canine, Guinea pig, Human, Mouse, Porcine, Rat
Rabbit
Polyclonal
Biotin
100 μlCSF1 Rabbit Polyclonal Antibody (Biotin) [orb2119369]
IHC, WB
Canine, Guinea pig, Human, Rabbit, Rat
Rabbit
Polyclonal
Biotin
100 μl

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To detect hM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human M-CSF (orb1272386) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hM-CSF.

To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
CSF1 Antibody (Biotin) (orb1272385)
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