M Cadherin (CDH15) Antibody (C-term)

• Catalog Number: orb1935884
• Category: Antibodies
• Description: M Cadherin (CDH15) Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P55291
• MW: 88916 Da
• Tested applications: IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CDH14, CDH3
• Research Area: Cell Biology, Infectious Disease & Virology, Neuro
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CDH15 (arrow) using rabbit polyclonal CDH15 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH15 gene (Lane 2).

Anti-CDH15 Antibody (C-term) at 1: 8000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 89 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with CDH15 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.