m-3M3FBS

SKU: orb1298856

Description

m-3M3FBS is a cell-permeable activator of phospholipase C (PLC). It is used in research to study PLC-mediated signaling pathways, including calcium mobilization and inositol phosphate production, in both cellular and biochemical assay systems.

Research Area

Cell Biology

Key Properties

• CAS Number: 200933-14-8
• MW: 343.37
• Purity: >98%
• Formula: C₁₆H₁₆F₃NO₂S
• SMILES: Cc1cc(C)c(c(C)c1)S(=O)(=O)Nc1cccc(c1)C(F)(F)F
• Target: Phospholipase
• Solubility: Soluble in DMSO (up to 50 mg/ml) or in Ethanol (up to 40 mg/ml).

Bioactivity

• In Vitro:
  m-3M3FBS stimulated a transient intracellular calcium concentration ([Ca(2+)](i)) increase in neutrophils. Moreover, m-3M3FBS stimulated the formation of inositol phosphates in U937 cells, indicating that it stimulates PLC activity. The compound showed no cell-type specificity in terms of [Ca(2+)](i) increase in the various cell lines including leukocytes, fibroblasts, and neuronal cells, also ruled out the possible involvement of heterotrimeric G proteins in m-3M3FBS-stimulated signaling by confirming the following: 1) pertussis toxin does not inhibit m-3M3FBS-induced [Ca(2+)](i) increase; 2) m-3M3FBS does not stimulate cyclic AMP generation; and 3) the inhibition of G(q) by the regulator of G protein-signaling 2 does not affect the m-3M3FBS-induced [Ca(2+)](i) increase. m-3M3FBS stimulated PLC activity in vitro. The purified isoforms of PLC that were tested (i.e., beta2, beta3, gamma1, gamma2, and delta1) were activated by m-3M3FBS and showed no isoform specificity.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cells, calcium, Apoptosis, m3M3FBS, m-3M3FBS, m-3M-3FBS, m 3M3FBS, PLC, neutrophils, inhibit, leukemic, inositol, Inhibitor, Phospholipase, phosphate, THP-1, superoxide, U937

Compound Identifiers

PubChem CID

761523