m-3M3FBS

SKU: orb1220031

Description

m-3M3FBS is a phospholipase C (PLC) activator. m-3M3FBS stimulated a transient intracellular calcium concentration ([Ca(2+)](i)) increase in neutrophils. Moreover, m-3M3FBS stimulated the formation of inositol phosphates in U937 cells, indicating that it stimulates PLC activity. The compound showed no cell-type specificity in terms of [Ca(2+)](i) increase in the various cell lines including leukocytes, fibroblasts, and neuronal cells, also ruled out the possible involvement of heterotrimeric G proteins in m-3M3FBS-stimulated signaling by confirming the following: 1) pertussis toxin does not inhibit m-3M3FBS-induced [Ca(2+)](i) increase; 2) m-3M3FBS does not stimulate cyclic AMP generation; and 3) the inhibition of G(q) by the regulator of G protein-signaling 2 does not affect the m-3M3FBS-induced [Ca(2+)](i) increase. m-3M3FBS stimulated PLC activity in vitro. The purified isoforms of PLC that were tested (i.e., beta2, beta3, gamma1, gamma2, and delta1) were activated by m-3M3FBS and showed no isoform specificity.

Key Properties

• CAS Number: 200933-14-8
• MW: 343.36
• Purity: >98% (HPLC)
• Formula: C₁₆H₁₆F₃NO₂S
• SMILES: Cc1cc(C)c(c(C)c1)S(=O)(=O)Nc1cccc(c1)C(F)(F)F
• Target: Phosphatase
• Solubility: In Vitro: DMSO : 100 mg/mL (291.24 mM)

Bioactivity

• In Vitro:
  m-3M3FBS (5-50 μM) stimulates the formation of inositol phosphates in U937 cells. m-3M3FBS (50 μM; 24 hours) inhibits the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes. m-3M3FBS (50 μM; 24 hours) induces U937 cell apoptosis. Cell Viability Assay Cell line: U937 and THP-1 cells. Concentration: 50 μM. Incubation time: 24 hours. Result: Inhibited the growth of the leukemic cell lines U937 and THP-1, but not primary monocytes. Cell Viability Assay Cell line: U937 cells. Concentration: 50 μM. Incubation time: 24 hours. Result: The apoptotic rate of m-3M3FBS-treated cells was 53.9%.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Phosphatase