LYZ Antibody (C-term)

• Catalog Number: orb1788455
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This LYZ antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from the C-terminal region of human LYZ.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: 119-154 aa
• UniProt ID: P61626
• MW: 16537 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:2000, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25, IHC-P: 1:25
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Lysozyme C,4-beta-N-acetylmuramidase C, LYZ, LZM
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded M. small intestine section using LYZ Antibody (C-term). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. small intestine section using LYZ Antibody (C-term). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. small intestine section using LYZ Antibody (C-term). Diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from HepG2, THP-1, WiDr cell line and human spleen tissue lysate (from left to right), using LYZ Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

All lanes: Anti-LYZ Antibody (C-term) at 1:2000 dilution. Lane 1: THP-1 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Human spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-LYZ Antibody (C-term) at 1:2000 dilution. Lane 1: WiDr whole cell lysate. Lane 2: THP-1 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: Human spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining LYZ in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.