LY6E Antibody

• Catalog Number: orb1249070
• Category: Antibodies
• Description: LY6E Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: SCQEKDHYCIT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments in Mouse Brain, Kidney, Heart and Spleen lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunohistochemistry:Customer obtained staining in Mouse Placenta in a pattern consistent with in situ hybridisation results, and no staining in KO mice. This antibody has been successfully used in IHC on Mouse: M. Hughes et al, Placenta. 2013 Sep;34(9):831-5. PMID: 23830620.Immunofluorescence: Strong expression of the protein seen in the membranes of NIH3T3 and L929 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: LY6E
• UniProt ID: Q64253
• NCBI: NP_032555.1, NP_002337.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: LY6E, lymphocyte antigen 6 complex, locus E, RIG-E
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments in Mouse Brain, Kidney, Heart and Spleen lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunohistochemistry:Customer obtained staining in Mouse Placenta in a pattern consistent with in situ hybridisation results, and no staining in KO mice. This antibody has been successfully used in IHC on Mouse: M. Hughes et al, Placenta. 2013 Sep;34(9):831-5. PMID: 23830620.Immunofluorescence: Strong expression of the protein seen in the membranes of NIH3T3 and L929 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249070 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI.

orb1249070 Immunofluorescence analysis of paraformaldehyde fixed L929 cells (Mouse fibroblast cell line), permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining.

orb1249070 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).