Lumican Rabbit Polyclonal Antibody

SKU: orb100104

Description

Lumican Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Equine, Human, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Lumican (61-160/338aa)
• Target: LUM
• Molecular Weight: 35 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Keratan sulfate proteoglycan lumican; KSPG lumican; LDC; LUM; LUM_HUMAN; Lumican; Lumican precursor; lumican proteoglycan; SLRR2D.

Paraformaldehyde-fixed, paraffin embedded (rat retina tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Lumican) Polyclonal Antibody, Unconjugated (orb100104) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

LUM

UniProt

P51884

References

SS Mahdavi, P Noohi, MJ Abdekhodaie, A Baradaran-Rafii, MH Nekoofar Development of photoreactive collagen-based bioinks for stereolithography 3D bioprinting Taylor & Francis, (2025)

PubMed:

Applications

IHC

Reactivity

Human