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Lumican Rabbit Polyclonal Antibody Discontinued

SKU: orb100104

Description

Lumican Rabbit Polyclonal Antibody

Research Area

Cancer Research, Signal Transduction

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution RangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityRat
Predicted ReactivityBovine, Equine, Human, Mouse, Porcine, Rabbit

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Lumican (61-160/338aa)
TargetLUM
Molecular Weight35 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Keratan sulfate proteoglycan lumican; KSPG lumican; LDC; LUM; LUM_HUMAN; Lumican; Lumican precursor; lumican proteoglycan; SLRR2D.

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Lumican Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat retina tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Lumican) Polyclonal Antibody, Unconjugated (orb100104) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

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SS Mahdavi, P Noohi, MJ Abdekhodaie, A Baradaran-Rafii, MH Nekoofar Development of photoreactive collagen-based bioinks for stereolithography 3D bioprinting Taylor & Francis, (2025)

Applications

IHC

Reactivity

Human

Lumican Rabbit Polyclonal Antibody (orb100104)

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