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LUXB_VIBFI, luxA Antibody
Description
Images & Validation
−| Tested Applications | DOT, ELISA, IHC, IP, WB |
|---|---|
| Dilution Range | ELISA: 1:5,000 - 1:25,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000 |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | Luciferase [Photobacterium fischerii] |
| Target | LUXB_VIBFI, luxA |
| Purity | This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Luciferase [Photobacterium fischerii]. Cross reactivity against Luciferase from other tissues and species may occur but have not been specifically determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 90 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 60 min at RT. Primary Antibody: Rabbit anti-Luciferase at 1 ug/ml 1hr RT. Secondary Antibody: Goat Anti-Rabbit IgG HRP orb347654 at 1:40000 for 30 min RT.
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Protocol Information
LUXB_VIBFI, luxA Antibody (orb750437)
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