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LUXB_VIBFI, luxA Antibody

SKU: orb750437

Description

luxA antibody

Images & Validation

Tested ApplicationsDOT, ELISA, IHC, IP, WB
Dilution RangeELISA: 1:5,000 - 1:25,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
Application Notes
Luciferase Antibody has been tested by dot blot and is suitable for western blotting, immunohistochemistry, immunoprecipitation, and ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeAntiserum
ImmunogenLuciferase [Photobacterium fischerii]
TargetLUXB_VIBFI, luxA
PurityThis product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Luciferase [Photobacterium fischerii]. Cross reactivity against Luciferase from other tissues and species may occur but have not been specifically determined.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/Preservatives0.01% (w/v) Sodium Azide
Concentration90 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

rabbit anti-Luciferase Antibody, Alkanal monooxygenase alpha chain, Bacterial luciferase alpha chain, luxA, Alkanal monooxygenase beta chain, Bacterial luciferase beta chain, luxB

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Quality Guarantee

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LUXB_VIBFI, luxA Antibody

Dot Blot results of Rabbit anti-Luciferase Antibody. Dots are Luciferase (Photobacterium fischerii) at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 60 min at RT. Primary Antibody: Rabbit anti-Luciferase at 1 ug/ml 1hr RT. Secondary Antibody: Goat Anti-Rabbit IgG HRP orb347654 at 1:40000 for 30 min RT.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP29238.1

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Datasheet
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
IP
Immunoprecipitation
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DOT
Dot Blot
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LUXB_VIBFI, luxA Antibody (orb750437)

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2 ml
$ 2,080.00
DispatchUsually dispatched within 5-10 working days
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