Luciferase antibody

• Catalog Number: orb344219
• Category: Antibodies
• Description: Luciferase antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Isotype: IgG
• Immunogen: Luciferase [Photinus pyralis (Firefly)]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:200 - 1:1000, IHC: 1:1500, IF: User Optimized, WB: 1:1000 - 1:5000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Luciferase Antibody, Luciferin 4-monooxy
• Note: For research use only
• Application notes: Anti-Luciferase Antibody has been tested in Western Blot, IHC, IF, and ELISA. Expect a band ~60kDa in appropriate cell lysates. Although not tested, this antibody would be useful in immunoprecipitation, immunocytochemistry, and most immunological methods requiring high titers and specificity.
• Expiration Date: 12 months from date of receipt.