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Catalog Number | orb334957 |
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Category | Antibodies |
Description | Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Insect |
Isotype | IgG |
Immunogen | Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli. |
Concentration | 3 mg/ml |
Dilution range | WB:1:500-1:2,000 |
Form/Appearance | Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum. |
Conjugation | Unconjugated |
Target | Luciferase |
Storage | Store at -20°C for long-term storage. Store at 2-8°C for up to one month. |
Buffer/Preservatives | PBS, 20% glycerol and 0.05% sodium azide |
Alternative names | Luc and Luciferin 4-monooxygenase antibody. Read more... |
Note | For research use only |
Application notes | The antibody solution should be gently mixed before use. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of staining of lentivirus expressing luciferase lysates using Luciferase antibody
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