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Catalog Number | orb1930498 |
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Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB20589 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:8000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 37192 Da |
Target | This LSP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-134 amino acids from the Central region of human LSP1. |
UniProt ID | P33241 |
NCBI | NP_001229861.1, NP_001013271.1, NP_001013273.1, NP_002330.1, NP_001013272.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Lymphocyte-specific protein 1, 47 kDa actin-bindin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
LSP1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
LSP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LSP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
LSP1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35 ug/lane). This demonstrates the LSP1 antibody detected the LSP1 protein (arrow).
All lanes: Anti-LSP1 Antibody (Center) at 1: 8000 dilution. Lane 1: Daudi whole cell lysate. Lane 2: Ramos whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 37 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.