LSD1 Antibody (C-term)

SKU: orb1937673

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB7583
• Immunogen: This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 819-852 amino acids from the C-terminal region of human LSD1. Antigen Region: 819-852 aa.
• Target: KDM1A (HGNC:29079)
• Molecular Weight: 92903 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lysine-specific histone demethylase 1A, 1---, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, KDM1A, AOF2, KDM1, KIAA0601, LSD1

Fluorescent confocal image of Hela cell stained with hLSD1-Y712. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hLSD1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). hLSD1 immunoreactivity is localized to nucleus significantly.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of AOF2 (arrow) using LSD1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2).

Western immunoblot. Nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted with anti-LSD1 Antibody. Nuclear LSD1 protein levels increased in carcinogen-treated HMEC compared with control HMEC.

Quick Database Links

Gene Symbol

KDM1A (HGNC:29079)

UniProt

O60341

RefSeq (Protein):NP_055828.2, NP_001009999.1