LRRK2/Dardarin Antibody: PerCP

• Catalog Number: orb150601
• Category: Antibodies
• Description: Mouse monoclonal to LRRK2 (PerCP). LRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N231B/34 (Formerly sold as S231B-34)
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Rat
• Isotype: IgG2a
• Immunogen: Fusion protein amino acids 841-960 of human LRRK2. 81% identical in mouse, 80% identical in rat. < 30% identity with LRRK1.
• Concentration: 1 mg/ml
• Dilution range: WB (1:100)
• Conjugation: PerCP
• MW: 200kDa
• Target: LRRK2
• Entrez: 120892
• UniProt ID: Q5S007
• NCBI: NP_940980
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Alternative names: Leucine-rich repeat kinase 2 antibody, RIP7 antibo
• Note: For research use only
• Application notes: 10 µg/ml of SMC-445 was sufficient for detection of LRRK2/Dardarin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRRK2/Dardarin Monoclonal Antibody, Clone N231B/34. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LRRK2/Dardarin Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRRK2/Dardarin Antibody (D) Composite.