LRRK2 Antibody (APC)

SKU: orb150596

Description

Mouse monoclonal to LRRK2 (APC). LRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features..

Research Area

Cell Biology

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:100)
• Reactivity: Human, Rat
• Application Notes:
  10 µg/ml was sufficient for detection of LRRK2/Dardarin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: N231B/34 (Formerly sold as S231B-34)
• Immunogen: Fusion protein amino acids 841-960 of human LRRK2. 81% identical in mouse, 80% identical in rat. < 30% identity with LRRK1.
• Target: LRRK2
• Molecular Weight: 200kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LRRK2, Leucine-rich repeat kinase 2, Leucine-rich repeat serine/threonine-protein kinase 2, RIP7, RIPK7, PARK8, Dardarin, ROCO 2, Augmented in rheumatoid arthritis 17

Quick Database Links

Gene Symbol

LRRK2

UniProt

Q5S007

NCBI Gene ID

120892

RefSeq (Protein):NP_940980