LRRC45 Antibody (C-term)

• Catalog Number: orb1938240
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This LRRC45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 615-643 amino acids from the C-terminal region of human LRRC45.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q96CN5
• MW: 75951 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:25, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Clone Number: RB29103
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Leucine-rich repeat-containing protein 45, LRRC45
• Note: For research use only
• NCBI: NP_659436.1

Immunofluorescent analysis of U251 cells, using LRRC45 Antibody (C-term). diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).

LRRC45 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

LRRC45 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of LRRC45 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of lysates from human blood plasma tissue (from left to right), using LRRC45 Antibody (C-term).diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary Antibody. Lysates at 35 ug per lane.