LRP5 Antibody

SKU: orb1247303

Description

LRP5 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 180kDa band observed in lysates of cell line HepG2 (calculated MW of 179kDa according to NP_002326.2). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver for this particular batch.Immunohistochemistry:In paraffin embedded Human Liver shows weak cytoplasm staining in some hepatocytes. Recommended concentration: 2-4µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-ERVEKTTGDKRT
• Target: LRP5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LRP5, low density lipoprotein receptor-related protein 5, HGNC:6697, BMND1, HBM, LR3, LRP7, OPPG, OPS, VBCH2, low density lipoprotein receptor-related protein 7, osteoporosis pseudoglioma syndrome, EVR1, EVR4, OPTA1, exudative vitreoretinopathy 1,

orb1247303 (1 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247303 (2 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Quick Database Links

Gene Symbol

LRP5

UniProt

O75197

RefSeq (Protein):NP_002326.2