LRP5 Antibody

• Catalog Number: orb1247303
• Category: Antibodies
• Description: LRP5 Antibody
• Target: LRP5
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-ERVEKTTGDKRT
• UniProt ID: O75197
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 180kDa band observed in lysates of cell line HepG2 (calculated MW of 179kDa according to NP_002326.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver for this particular batch.Immunohistochemistry:In paraffin embedded Human Liver shows weak cytoplasm staining in some hepatocytes. Recommended concentration: 2-4ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LRP5, low density lipoprotein receptor-related pro
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_002326.2

orb1247303 (1 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247303 (2 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.