LRP2/Megalin Antibody

SKU: orb1537357

Description

LRP2/Megalin Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: IHC-P (10 µg/ml), WB (1:500 - 1:2000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Further information: Western blot analysis of LRP2 expression in mouse lung (A) whole cell lysates. Immunohistochemical analysis of LRP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant full length protein of human LRP2
• Target: LRP2 / Megalin
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LRP2, DBS, Glycoprotein 330, gp330, Megalin, LRP-2, Calcium sensor protein

WB analysis of orb1537357.

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Quick Database Links

Gene Symbol

LRP2 / Megalin

Manuel Johanns, Pascale Lemoine, Virginie Janssens, Giuseppina Grieco, Soren K. Moestrup, Rikke Nielsen, Erik I. Christensen, Pierre J. Courtoy, Hervé Emonard, Etienne Marbaix, Patrick Henriet Cellular uptake of proMMP-2:TIMP-2 complexes by the endocytic receptor megalin/LRP-2 Sci Rep, 7, 1 (2017)

PubMed: 28659595

Applications

WB

Reactivity

Rat