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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB (1:1000); IHC (1:50) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide from the N-terminal of Human Low-density lipoprotein receptor-related protein 1 (aa. 160-260) |
| Target | LRP1 |
| Molecular Weight | 80 kDa |
| Purification | Peptide Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−LRP1, LRP-1, LRP-85, LRP-515, Alpha-2-macroglobulin receptor, A2MR, Apolipoprotein E receptor, Prolow-density lipoprotein receptor-related protein 1, CD91, LRPICD, APOER, APR
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Immunohistochemistry analysis using Rabbit Anti-LRP1 Polyclonal Antibody. Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-LRP1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western blot analysis of Mouse, Rat Brain showing detection of 504.6 kDa LRP-1 protein using Rabbit Anti-LRP-1 Polyclonal Antibody. Load: 15 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-LRP-1 Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 504.6 kDa. Other Band (s): 80 kDa.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002323.2 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Filter by Applications
Filter by Species
Manuel Johanns et al. Cellular uptake of proMMP-2:TIMP-2 complexes by the endocytic receptor megalin/LRP-2 Sci Rep, 7(1), 4328 (2017)
Applications
WB
Reactivity
Rat
LRP1 Antibody (orb535874)
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