LOXL4 Antibody (C-term)

SKU: orb1934194

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:2000, FC - 1:25
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This LOXL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-603 amino acids from the C-terminal region of human LOXL4. Antigen Region: 575-603 aa.
• Target: LOXL4
• Molecular Weight: 84483 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lysyl oxidase homolog 4, 143-, Lysyl oxidase-like protein 4, Lysyl oxidase-related protein C, LOXL4, LOXC

LOXL4 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35 ug/lane). This demonstrates the LOXL4 antibody detected the LOXL4 protein (arrow).

All lanes: Anti-LOXL4 Antibody (Center) at 1:2000 dilution. Lane 1: Human testis lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing A549 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

LOXL4

UniProt

Q96JB6

RefSeq (Protein):NP_115587.6