Losatuxizumab Antibody

SKU: orb1980374

Description

Losatuxizumab (ABT-806) is the unconjugated, naked monoclonal antibody precursor to the antibody-drug conjugate Losatuxizumab vedotin. It targets EGFR and is utilized in oncology research for studying advanced solid tumors, with applications in both in vitro binding assays and in vivo xenograft models.

Research Area

Cardiovascular Research, Signal Transduction

Key Properties

• CAS Number: 1801544-27-3
• MW: 144.85 kDa
• Purity: 95.00%
• Target: EGFR

Bioactivity

• Target IC50:
  EGFR (1-501):0.66 nM (EC50)|EGFR (WT):0.96 nM (EC50)|EGFR vIII:0.12 nM (EC50)|EGFR phosphorylation:1.2 nM
• In Vivo:
  Losatuxizumab exhibits antitumor activity against multiple wild-type EGFR-expressing squamous cell carcinoma xenograft models. Losatuxizumab (40 mg/kg, intraperitoneal) significantly delayed the growth of U87MGde2-7 tumors in mice. Losatuxizumab (10 and 40 mg/kg, intraperitoneal) prolonged survival in a patient-derived xenograft glioblastoma (PDX GBM) model (SN0207 tumors) while decreasing pEGFR levels in tumor tissue.
• In Vitro:
  Losatuxizumab (ABT-806) binds EGFR with EC50 values of 0.96 nM for EGFR wild type, 0.09 nM for EGFR(C271A,C283A), 0.12 nM for EGFR(vIII), and 0.66 nM for EGFR(1-501). Losatuxizumab inhibited EGF-induced EGFR phosphorylation with an IC50 of 1.2 nM in the NR6 huEGFR(C271A,C283A) cell line.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ABT806, ABT-806

Quick Database Links

Gene Symbol

EGFR