LMO4 Antibody (Center)

• Catalog Number: orb1938728
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This LMO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-131 amino acids from the Central region of human LMO4.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P61968
• MW: 17994 Da
• Tested applications: FC, IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB24682
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: LIM domain transcription factor LMO4, Breast tumor
• Note: For research use only
• NCBI: NP_006760.1

Fluorescent image of A549 cell stained with LMO4 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LMO4 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). LMO4 immunoreactivity is localized to Nucleus significantly.

LMO4 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

LMO4 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35 ug/lane). This demonstrates the LMO4 antibody detected the LMO4 protein (arrow).