LMO2 Antibody / Rhombotin 2

SKU: orb2643507

Description

The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. It has a particular function in normal and lymphatic endothelial cells involving the regulation of angiogenesis and lymph-angiogenesis. Immunohistochemical studies have also demonstrated expression of LMO2 in both normal germinal center B-cells and germinal center-derived B-cell lymphomas, including follicular lymphoma and diffuse large B-cell lymphoma.The use of anti-LMO2 is valuable as a tool in the identification of lymphomas of B-cell origin. LMO2 is useful in differentiating follicular lymphoma (LMO2+) from nodal marginal zone lymphoma (LM02-). It also is positive in Hodgkin s and Burkitt s lymphomas.

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells in 0.1ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the LMO2 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: LMO2/1971
• Immunogen: A recombinant human partial protein (amino acids 23-140) was used as the immunogen for the LMO2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human Ramos cell lysate with LMO2 antibody. Predicted molecular weight ~25 kDa.

Flow cytometry testing of permeabilized human K562 cells with LMO2 antibody; Red=isotype control, Blue= LMO2 antibody.

IHC staining of FFPE human follicular lymphoma with LMO2 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human pancreas with LMO2 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free LMO2 antibody as confirmation of integrity and purity.

Quick Database Links

UniProt

P25791