LJH685

SKU: orb1300384

Description

LJH685 is a potent and selective pan-RSK inhibitor, exhibiting IC50 values of 6, 5, and 4 nM against RSK1, RSK2, and RSK3, respectively. This small molecule is a valuable research tool for studying RSK signaling in cancer cell proliferation and survival, with applications in both in vitro and in vivo models.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 1627710-50-2
• MW: 381.42
• Purity: 99.78% (May vary between batches)
• Formula: C₂₂H₂₁F₂N₃O
• SMILES: CN1CCN(CC1)c1ccc(cc1)-c1ccncc1-c1cc(F)c(O)c(F)c1
• Target: Apoptosis,S6 Kinase
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:71 mg/mL (186.15 mM);Ethanol:16 mg/mL (41.95 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.24 mM)

Bioactivity

• Target IC50:
  RSK3:4 nM|RSK1:6 nM|RSK2:5 nM
• In Vitro:
  LJH685 modulates YB1 phosphorylation by potently and selectively inhibiting RSK in cells. In MAPK pathway–dependent cancer cell lines, LJH685 shows antiproliferative effects, and causes cell-cycle regulation and apoptosis induction.
• Cell Research:
  Cell growth under attached conditions is assessed by plating 1000 cells per well on 96-well tissue culture-treated plates in cell growth medium. Appropriate dilutions of compound are added medium above cells and cell growth is assessed after 72 hrs by addition of CellTiter Glo reagent according to manufacturer's directions.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, LJH 685, LJH685, LJH-685, inhibit, Apoptosis, Ribosomal S6 Kinase (RSK), RSK1, RSK3, RSK2, S6 Kinase, S6K, S6Kinase

Compound Identifiers

PubChem CID

73010393