LIPA/Lysosomal Acid Lipase Antibody

• Catalog Number: orb1535659
• Category: Antibodies
• Description: LIPA/Lysosomal Acid Lipase Antibody
• Target: LIPA / Lysosomal Acid Lipase
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b,k
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: PBS, pH 7.4. PBS, pH 7.4
• Purification: Purified from ascites by Protein A
• Immunogen: LIPA (AAH12287, aa 1-399). A full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Tested applications: ELISA, IHC, IHC-P, IP, WB
• Dilution range: ELISA, IHC, IHC-P (10 µg/ml), IP, WB
• Application notes: Further information: Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
• Clone Number: 1F9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LIPA, Cholesterol ester hydrolase, Cholesteryl est
• Note: For research use only

Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.Lane 1: LIPA transfected lysate (45 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA rabbit polyclonal antibody.

Detection limit for recombinant GST tagged LIPA is approximately 10 ng/ml as a capture antibody.