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| Catalog Number | orb1239570 |
|---|---|
| Category | Antibodies |
| Description | LIN54 Antibody |
| Target | LIN54 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1 mg/mL |
| Buffer/Preservatives | LIN54 antibody is supplied in PBS containing 0.02% sodium azide. |
| Purification | LIN54 antibody is affinity chromatography purified via peptide column. |
| Immunogen | LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54.The immunogen is located within the last 50 amino acids of LIN54. |
| UniProt ID | Q6MZP7 |
| MW | Predicted: 88 kDa Observed: 82 kDa |
| Tested applications | ELISA, ICC, IF, WB |
| Application notes | LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 2.5 μg/mL. For immunofluorescence start at 20 μg/mLAntibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | LIN-54, JC8.6, CXCDC1, MIP120 |
| Research Area | Cell Biology |
| Note | For research use only |
| NCBI | NP_919258 |

Western blot analysis of LIN54 in HeLa cell lysate with LIN54 antibody at 1 µg/ml.

Immunocytochemistry of LIN54 in HeLa cells with LIN54 at 2.5 µg/mL.

Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 µg/ml.

Immunocytochemistry of LIN54 in HeLa cells with LIN54 antibody at 10 µg/ml.

Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 µg/ml. Red: LIN54 Antibody (orb1239570) Blue: DAPI staining.
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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