LIN54 Antibody

SKU: orb1239570

Description

LIN54 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 2.5 μg/mL. For immunofluorescence start at 20 μg/mLAntibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54.The immunogen is located within the last 50 amino acids of LIN54.
• Target: LIN54
• Molecular Weight: Predicted: 88 kDa Observed: 82 kDa
• Purification: LIN54 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: LIN54 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LIN-54, JC8.6, CXCDC1, MIP120

Western blot analysis of LIN54 in HeLa cell lysate with LIN54 antibody at 1 µg/ml.

Immunocytochemistry of LIN54 in HeLa cells with LIN54 at 2.5 µg/mL.

Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 µg/ml.

Immunocytochemistry of LIN54 in HeLa cells with LIN54 antibody at 10 µg/ml.

Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 µg/ml. Red: LIN54 Antibody (orb1239570) Blue: DAPI staining.

Quick Database Links

Gene Symbol

LIN54

UniProt

Q6MZP7

RefSeq (Protein):NP_919258