LIN28B Antibody [Knockout Validated]

SKU: orb1787816

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:4000, WB: 1:500-1:2000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Target: This LIN28B antibody is generated from a mouse immunized with a recombinant protein of human LIN28B.
• Molecular Weight: 27084 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2

All lanes: Anti-LIN28B Antibody at 1:4000 dilution. Lane 1: 293 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

A single 34.3 kDa band for the HEK293T wild type lysate was observed (3 ug/ml anti- LIN28B) that closed matches the predicted size of 27.1 kDa. The molecular weight discrepancy could be due a post-translationally modified form of the target protein, a splice-variant form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A single 60.7 kDa band was observed in the knock out lysate (20 ug lane only).

Quick Database Links

Gene Symbol

This LIN28B antibody is generated from a mouse immunized with a recombinant protein of human LIN28B.

UniProt

Q6ZN17