LIM and SH3 domain protein 1 rabbit pAb

• Catalog Number: orb771692
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LIM and SH3 domain protein 1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: Synthesized peptide derived from human LIM and SH3 domain protein 1. at AA range: 90-130
• MW: 38
• Tested applications: ELISA, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-LIM and SH3 domain protein 1 (LASP-1) (Metast
• Note: For research use only

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night.

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).