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Description
Research Area
Epigenetics, Immunology
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB 1:500 - 1:2000IHC 1:50 - 1:200IF 1:50 - 1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 22-259 of human LILRB4 (NP_001265355.2). |
| Molecular Weight | 49kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−LILRB4, CD85K, ILT-3, ILT3, LIR-5, LIR5
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Immunofluorescence analysis of paraffin-embedded mouse spleen using LILRB4 Rabbit pAb (orb1150417) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using LILRB4 Rabbit pAb (orb1150417) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using LILRB4 Rabbit pAb (orb1150417) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Western blot analysis of various lysates using LILRB4 Rabbit pAb (orb1150417) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot analysis of various lysates using LILRB4 Rabbit pAb (orb1150417) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Quick Database Links
UniProt
UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Yilin Ma # 1, Kai Zheng # 1 2 3, Chengcheng Zhao 1, Jieli Chen 1 2 3, Lin Chen 4, Yue Zhang 1, Tao Chen 1, Xiuhua Yao 1 2 3, Ying Cai 5 6 7, Jialing Wu Microglia LILRB4 upregulation reduces brain damage after acute ischemic stroke by limiting CD8+ T cell recruitment J Neuroinflammation, (2024)
Applications
WB
Reactivity
Mouse
LILRB4 Rabbit pAb (orb1150417)
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