LIG3 Antibody

• Catalog Number: orb1260387
• Category: Antibodies
• Description: LIG3 Antibody
• Target: LIG3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 730-1009 of human LIG3 (NP_039269.2).
• UniProt ID: P49916
• MW: Observed: 112kDa
• Tested applications: IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DNA ligase 3, DNA ligase III, LIG2
• Note: For research use only

Western blot analysis of extracts of various cell lines, using LIG3 antibody (orb1260387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat heart using LIG3 antibody (orb1260387) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human oophoroma using LIG3 antibody (orb1260387) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using LIG3 antibody (orb1260387) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using LIG3 antibody (orb1260387) at dilution of 1:100 (40x lens).