Licochalcone D

SKU: orb1222693

Description

Licochalcone D may be a potential drug for human melanoma treatment by inhibiting proliferation, inducing apoptosis via the mitochondrial pathway and blocking cell migration and invasion. Licochalcone D has cardioprotective potential against myocardial ischemia/reperfusion injury in langendorff-perfused rat hearts. Licochalcone D has anti-inflammatory activity, it shows suppression ability of nitric oxide (NO) production. Licochalcone D has anti-allergic activity, it suppresses degranulation by decreasing the intracellular Ca2+ level and tyrosine phosphorylation of ERK in RBL-2H3 cells.

Key Properties

• CAS Number: 144506-15-0
• MW: 354.4
• Purity: >98% (HPLC)
• Formula: C₂₁H₂₂O₅
• SMILES: COc1c(O)c(O)ccc1\C=C\C(=O)c1ccc(O)c(CC=C(C)C)c1
• Target: p38 MAPK
• Solubility: In Vitro: DMSO : 125 mg/mL (352.71 mM)

Bioactivity

• In Vivo:
  Animal model: C57BL/6 mice, B16F0 tumor model. Dosage: 25 and 50 mg/kg. Administration: Intragastric administration, daily for a week. Result: Obviously lowered the tumor growth rates, the tumor growth inhibition rates were calculated to be 32.0 and 54.1% at 25 and 50 mg/kg, respectively.
• In Vitro:
  Western blot analysis. Cell line: RAW264.7 cells Concentration: 10 μM. Incubation time: 1 h or 24 h (iNOS), following LPS stimulation (1 μg/mL) for 30 min. Result: Inhibited the phosphorylation of NF-κB at serine 276 but not at serine 536.Inhibited the LPS-induced expression of TNFα mRNA and MCP-1 mRNA. Strongly inhibited the expression of iNOS. Cell Viability Assay Cell line: A375 cells or SK-MEL-5. Concentration: 0, 1, 2.5, 5, 15, 30, 45, 60, 75 and 90 μM (A375) or 0, 20, 40, 60 and 80 μM (SK-MEL-5) Incubation time: 24 h. Result: Inhibited the proliferation of A375 and SK-MEL-5 in a concentration dependent manner. Apoptosis Analysis Cell line: A375. Concentration: 0, 30, 60 and 90 μM. Incubation time: 24 h. Result: Cells exhibited obvious apoptotic characteristics after the treatment. RT-PCR Cell line: A375. Concentration: 0, 30, 60 and 90 μM. Incubation time: 24 h. Result: Downregulated the mRNA level of Bcl-2 and upregulated the mRNA levels of caspase-3, caspase-9 and Bax. Cell Invasion Assay Cell line: A375. Concentration: 25 μM. Incubation time: 24 h. Result: Significantly suppressed cell invasion. Cell Migration Assay Cell line: A375. Concentration: 25 μM. Incubation time: 24 h. Result: Significantly suppressed cell migration in the A375 cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Licochalcone D | Licochalcone-D

Quick Database Links

Gene Symbol

p38 MAPK