LHCGR Antibody

SKU: orb1247316

Description

LHCGR Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 35kDa band in K562 cell lysates after 0.1µg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we cannot currently find an explanation in the literature for this band, given the calculated size of 78.6kDa according to NP_000224.2.Immunofluorescence: Strong expression of the protein seen in the plasma membranes of HeLa cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CQGTALLDKTRYTE
• Target: LHCGR
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LHCGR, luteinizing hormone/choriogonadotropin receptor , LCGR, LGR2, LHR, hLHR , luteinizing hormone receptor, lutropin/choriogonadotropin receptor, LH receptor, CG receptor

orb1247316 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane staining.

orb1247316 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

LHCGR

UniProt

P22888

RefSeq (Protein):NP_000224.2