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LHCGR Antibody

SKU: orb1247316

Description

LHCGR Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, FC, IF
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 35kDa band in K562 cell lysates after 0.1µg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we cannot currently find an explanation in the literature for this band, given the calculated size of 78.6kDa according to NP_000224.2.Immunofluorescence: Strong expression of the protein seen in the plasma membranes of HeLa cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: CQGTALLDKTRYTE
TargetLHCGR
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

LHCGR, luteinizing hormone/choriogonadotropin receptor , LCGR, LGR2, LHR, hLHR , luteinizing hormone receptor, lutropin/choriogonadotropin receptor, LH receptor, CG receptor

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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