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Catalog Number | orb1933586 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB20572 |
Tested applications | WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:2000, WB: 1:1000, WB: 1:2000, WB: 1:2000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 35808 Da |
Target | This LGALS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-298 amino acids from the C-terminal region of human LGALS8. |
UniProt ID | O00214 |
NCBI | NP_006490.3, NP_963837.1, NP_963839.1, NP_963838.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Galectin-8, Gal-8, Po66 carbohydrate-binding prote Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
LGALS8 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35 ug/lane). This demonstrates the LGALS8 antibody detected the LGALS8 protein (arrow).
Anti-LGALS8 Antibody (C-term) at 1:2000 dilution + LNCaP whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-LGALS8 Antibody (C-term) at 1:2000 dilution. Lane 1: PC-3 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-LGALS8 Antibody (C-term) at 1:2000 dilution. Lane 1: LNCaP whole cell lysates. Lane 2: MDA-MB-231 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.