Lep Antibody

SKU: orb1272416

Description

Lep Antibody

Research Area

Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant murine Leptin.
• Target: Lep
• Purification: Anti-murine Leptin specific antibody was purified by affinity chromatography employing immobilized murine Leptin matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ob, obese, Ob, Leptin, Obesity factor,

To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine Leptin (orb1272415) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.

To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine cecum and large intestine tissue. The primary antibody was incubated at 0.20 ug/ml overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used.

This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine cecum and large intestine tissue. The primary antibody was incubated at 0.20 ug/ml overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used.

Quick Database Links

Gene Symbol

Lep

UniProt

P41160

RefSeq:P41160