Leonurine

SKU: orb1301257

Description

Leonurine

Research Area

Cell Biology

Key Properties

• CAS Number: 24697-74-3
• MW: 311.33
• Purity: 98.00%
• Formula: C₁₄H₂₁N₃O₅
• SMILES: Cl.COC1=CC(=CC(OC)=C1O)C(=O)OCCCCNC(N)=N
• Target: Autophagy
• Solubility: DMSO:3.12 mg/mL (10.02 mM)

Bioactivity

• In Vivo:
  Administration of leonurine (60 mg/kg) for 4 weeks significantly alleviated depression-like behaviors of chronic mild stress mice, including increased sucrose preference and reduced immobility time in forced swimming test and tail suspension test. We further found that leonurine (60 mg/kg) effectively restore the levels of 5 hydroxytryptamine, noradrenaline, and dopamine in the hippocampus and prefrontal cortex of chronic mild stress mice, accompanied by amelioration of hippocampal neuronal damage. furthermore , leonurine (60 mg/kg) significantly inhibite the production of proinflammatory cytokine interleukin-1β, interleukin-6 and TNF-α, and suppresse the nuclear factor kappa B signaling pathwa.
• In Vitro:
  Leonurine significantly inhibited lipid accumulation and promoted 3H-cholesterol efflux in human THP-1 macrophage-derived foam cells in a concentration dependent manner. Besides, bot the mRNA and protein levels of ABCA1/G1, PPARγ and LXRα were enhanced by leonurine, which were attenuated by LXRα siRNA or PPARγ siRNA transfectio.
• Cell Research:
  Human THP-1 cells were fully differentiated into foam cells b the pre-treatment with phorbol-12-myristate-13-acetate (PMA) and oxidized density lipoproteins (ox-LDL). After cells were incubated with various concentration of leonurine, Oil Red O staining and high-performance liquid chromatography (HPLC) assays were utilized to detect cellular lipid accumulation and cholesterol content, respectively Cellular cholesterol efflux was determined by liquid scintillation counting the mRNA and protein levels of ATP-binding cassette transporter A1/G1 (ABCA1/G1), peroxisome proliferator-activated receptor γ (PPARγ) and liver X receptor α (LXRα) in foam cells were assessed using real-time quantitative PCR (RT-qPCR) and western blot analyses, respectively Plasma triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C) levels in apoE-/- mice were evaluated using enzymatic methods the atherosclerotic lesion sizes and collagen contents in aortic roots were determined by Oil Red O and Masson's trichrome staining, respectivel.
• Animal Research:
  Use the chronic mild stress mouse model to explor the antidepressant effects of leonurine an the potential mechanisms. Behavioral tests including sucrose preference test, forced swimming test, and tail suspension test were taken to evaluate depression symptoms. Moreover the contents of monoamine neurotransmitters in hippocampus and prefrontal cortex were measured by high-performance liquid chromatography. Neuronal morphology was detected by transmission electron microscop.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Autophagy, Inhibitor, Leonurine, inhibit, SCM 198, SCM198, SCM-198

Compound Identifiers

PubChem CID

46837042