C6orf58 Antibody (Center)

• Catalog Number: orb1939204
• Category: Antibodies
• Description: C6orf58 Antibody (Center)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q6P5S2
• MW: 37926 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Cell Biology
• Note: For research use only

Anti-LEG1 Antibody (Center) at 1:1000 dilution + human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

LEG1 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

LEG1 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LEG1 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

LEG1 Antibody (Center) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the LEG1 antibody detected the LEG1 protein (arrow).