LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF1 alpha

• Catalog Number: orb2635789
• Category: Antibodies
• Description: Lymphoid enhancer-binding factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment (within amino acids 1-200) of human LEF1 protein was used as the immunogen for the recombinant LEF1 antibody.
• UniProt ID: Q9UJU2
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant LEF1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: LEF1/9403R
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant LEF1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil tissue with recombinant LEF1 antibody (clone LEF1/9403R). Strong nuclear staining of non-germinal center of cells observed. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant recombinant LEF1 antibody (clone LEF1/9403R) as confirmation of integrity and purity.