LC3 Antibody (APG8A/B)

SKU: orb1933543

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:500, IHC-P - 1:50-100
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from human LC3. Antigen Region: 23-52 aa.
• Target: MAP1LC3B (HGNC:13352)
• Molecular Weight: 14688 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using APG8a/b (MAP1LC3A/B) Antibody (Y38) (upper) or Beta-actin (lower).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

MAP1LC3B (HGNC:13352)

UniProt

Q9GZQ8

RefSeq (Protein):NP_073729.1