LC3 Antibody (P45)

SKU: orb1933560

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Microbiology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:100, WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-56 amino acids from human LC3. Antigen Region: 30-56 aa.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A

Western blot analysis of LC3 (APG8a) (arrow) using purified Pab. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LC3 (APG8a) gene (Lane 2).

Formalin-fixed and paraffin-embedded heart muscle tissue reacted with Autophagy LC3 APG8a (MAP1LC3A) Antibody (P45), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent image of U251 cells stained with LC3 (APG8A) (P45) antibody. U251 cells were treated with Chloroquine (50 μM, 16 h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with LC3 (APG8A) (P45) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Quick Database Links

UniProt

Q9H492, Q9GZQ8

RefSeq (Protein):NP_852610.1, NP_115903.1