LB100

SKU: orb1302850

Description

LB-100 is a cell-permeable, water-soluble inhibitor of protein phosphatase 2A (PP2A). It is widely used in cancer research to study cell cycle regulation, apoptosis, and DNA damage response in both cellular and animal models.

Research Area

Metabolism Research

Key Properties

• CAS Number: 1632032-53-1
• MW: 268.31
• Purity: 98.63% (May vary between batches)
• Formula: C₁₃H₂₀N₂O₄
• SMILES: C(=O)(C1[C@]2(O[C@@](C1C(O)=O)(CC2)[H])[H])N3CCN(C)CC3
• Target: Phosphatase
• Solubility: DMSO:< 1 mg/mL (insoluble or slightly soluble);H2O:67.5 mg/mL (251.57 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  PP2A(BxPc-3):0.85 μM|PP2A(Panc-1):3.87 μM
• In Vivo:
  Compared to the control group, LB-100 significantly increased the intracellular concentration of doxorubicin by approximately 2.5 times, enhancing the tumor cells' sensitivity to doxorubicin. Additionally, LB-100 amplified the secretion of vascular endothelial growth factor, thereby promoting angiogenesis mediated by HIF-1α-VEGF. LB-100 also demonstrated a notable inhibitory effect on the growth of BxPc-3 (IC50: 2.3 μM) and Panc-1 (IC50: 1.7 μM) cells. Following treatment with LB-100, a reduction of 30-50% in PP2A activity was observed in BxPc-3, SW1990, and Panc-1 tumor cells.
• In Vitro:
  In xenograft mouse models of pancreatic cancer, LB-100 (2 mg/kg, i.p.) enhances the therapeutic efficacy of the chemotherapy agent doxorubicin. LB-100 increases both the blood flow velocity on the tumor surface and the microvascular density of the tumor.
• Cell Research:
  Cytotoxicity is conducted by using a Cell Counting Kit-8. Cells are seeded in 96-well plates with a density of 3000 cells per well and are assessed after treatments following the CCK-8 protocol. Relative cytotoxicity is expressed as a percentage of speci c controls. (Only for Reference)

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PP2A, LB 100, LB100, LB-100

Compound Identifiers

PubChem CID

45101433