LAT1-IN-1

SKU: orb1218503

Description

LAT1-IN-1 (BCH) significantly inhibit cellular uptake of amino acids and mTOR phosphorylation, which induces the suppression of cancer growth and apoptosis. is a selective and competitive inhibitor of large neutral amino acid transporter 1 (LAT1).

Key Properties

• CAS Number: 20448-79-7
• MW: 155.19
• Purity: >98% (HPLC)
• Formula: C₈H₁₃NO₂
• SMILES: C1CC2CC1CC2(C(=O)O)N
• Target: Apoptosis
• Solubility: H2O:12.5 mg/mL (80.55 mM; Need ultrasonic);DMSO:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• In Vivo:
  BCH (200 mg/kg; intravenous injection; daily; for 14 days; male BALB/c nude mice) treatment significantly delays tumor growth and decreases glucose metabolism, indicating that LAT1 inhibition potentially suppresses esophageal cancer growth In vivo. Animal model: Male BALB/c nude mice (5-week-old) with KYSE150 cells. Dosage: 200 mg/kg. Administration: Intravenous injection; daily; for 14 days. Result: Significantly delayed tumor growth and decreased glucose metabolism.
• In Vitro:
  BCH (1-100 mM; 3 days; KYSE30 and KYSE150 esophageal cancer cells) treatment suppresses cell proliferation in a dose-dependent manner. BCH (30 mM; 24 and 48 hours; KYSE30 and KYSE150 cells) treatment significantly increases cell population in the G0/G1 phase in both KYSE30 and KYSE150 cells, indicating that BCH induces cell cycle arrest at G1 phase. BCH (30 mM; 0-24 hours; KYSE30 and KYSE150 cells) treatment decreases phosphorylation of 4E-BP1 and p70S6K at 30 minutes and the decrease is continued for 24 hours. The amount of mTOR, 4E-BP1, and p70S6K proteins is slightly decreased. Cell Proliferation Assay Cell line: KYSE30 and KYSE150 esophageal cancer cells. Concentration: 1 mM, 3 mM, 5 mM, 10 mM, 20 mM, 30 mM, 40 mM, 50 mM, or 100 mM. Incubation time: 3 days. Result: Cell proliferation was suppressed in a dose-dependent manner. Cell Cycle Analysis Cell line: KYSE30 and KYSE150 cells. Concentration: 30 mM. Incubation time: 24 and 48 hours. Result: Cell cycle arrest. Western blot analysis. Cell line: KYSE30 and KYSE150 cells. Concentration: 30 mM. Incubation time: 0 hour, 0.5 hour, 1 hour, 3 hours, 6 hours, 24 hours Result: Phosphorylation of 4E-BP1 and p70S6K was decreased. The amount of mTOR, 4E-BP1, and p70S6K proteins was slightly decreased.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BCH

Quick Database Links

Gene Symbol

Apoptosis