LAR Antibody (RPE)

SKU: orb167668

Description

Mouse monoclonal antibody to PTPRF (RPE ). PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function . Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of LAR/PTPRF in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: N165/38 (Formerly sold as S165-38)
• Immunogen: Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. > 80% identity with PTPRD and PTPRS. > 50% identity with PTPRM and PTPRK.
• Target: LAR
• Molecular Weight: 85kDa
• Purification: Protein G Purified
• Conjugation: RPE

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PTPRF, LAR, Leukocyte antigen related, Leukocyte antigen related PTP receptor, Leukocyte antigen related tyrosine phosphatase, Leukocyte common antigen related, Receptor-type tyrosine-protein phosphatase F, Receptor type tyrosine protein phosphatase F precursor, Protein Tyrosine Phosphatase Receptor Type F, Protein tyrosine phosphatase receptor type F polypeptide, Receptor linked phosphatase LAR, LAR protein, LARS, LARFN5C, LCA homolog, FLJ43335, FLJ45062, FLJ45567

Quick Database Links

Gene Symbol

LAR

UniProt

P10586

NCBI Gene ID

5792

RefSeq (Protein):NP_002831.2