LAR Antibody (APC)

SKU: orb167662

Description

Mouse monoclonal antibody to PTPRF (APC ). PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function . Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.

Research Area

Disease Biomarkers, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-443 was sufficient for detection of LAR/PTPRF in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: N165/38 (Formerly sold as S165-38)
• Immunogen: Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. > 80% identity with PTPRD and PTPRS. > 50% identity with PTPRM and PTPRK.
• Target: LAR
• Molecular Weight: 85kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PTPRF, LAR, Leukocyte antigen related, Leukocyte antigen related PTP receptor, Leukocyte antigen related tyrosine phosphatase, Leukocyte common antigen related, Receptor-type tyrosine-protein phosphatase F, Receptor type tyrosine protein phosphatase F precursor, Protein Tyrosine Phosphatase Receptor Type F, Protein tyrosine phosphatase receptor type F polypeptide, Receptor linked phosphatase LAR, LAR protein, LARS, LARFN5C, LCA homolog, FLJ43335, FLJ45062, FLJ45567

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAR/PTPRF Monoclonal Antibody, Clone N165/38. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LAR/PTPRF Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LAR/PTPRF Antibody (D) Composite.

Western Blot analysis of Rat Brain Membrane showing detection of LAR protein using Mouse Anti-LAR Monoclonal Antibody, Clone N165/38. Primary Antibody: Mouse Anti-LAR Monoclonal Antibody at 1:250.

Quick Database Links

Gene Symbol

LAR

UniProt

P10586

NCBI Gene ID

5792

RefSeq (Protein):NP_002831.2