LAMP2 Antibody

SKU: orb1822484

Description

Rat Anti-Mouse LAMP2 Monoclonal IgG1

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IP, WB
• Dilution Range: WB (1:1000), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
• Reactivity: Human, Mouse, Rabbit
• Application Notes:
  1 µg/ml of SMC-141 was sufficient for detection of LAMP2 in 20 µg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: GL2A7
• Immunogen: Purified preparation of mouse liver lysosomal membranes
• Target: LAMP2
• Molecular Weight: 100-110kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LAMP2, LAMP-2, Lamp2C, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2, CD107b, CD107 antigen-like family member B, Lysosomal membrane glycoprotein type B, LGP-B, Igp110, Igp2, LampB, MAC3

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-LAMP2 Monoclonal Antibody, Clone GL2A7. Tissue: Corneal Endothelial Cell (CEC). Species: Rabbit. Primary Antibody: Rat Anti-LAMP2 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).

Western Blot analysis of Human, Mouse HEK293 and 3T3NIH cell lysates showing detection of ~100-110 kDa LAMP2 protein using Rat Anti-LAMP2 Monoclonal Antibody, Clone GL2A7. Lane 1: MW ladder. Lane 2: Human HEK293 lysate (20 μg). Lane 3: Mouse 3T3NIH lysate (10 μg). Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rat Anti-LAMP2 Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rat at 1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~100-110 kDa.

Quick Database Links

Gene Symbol

LAMP2

UniProt

P17047

NCBI Gene ID

16784

RefSeq (Protein):NP_001017959.1