Laminin Antibody

SKU: orb345360

Description

Laminin antibody

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:4,000 - 1:8,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:500 - 1:,000
• Reactivity: Human
• Application Notes:
  Anti-Laminin (RABBIT) Antibody has been tested by IHC and was assayed by immunoblot and found to be reactive against Laminin at a dilution of 1:5,000 to 1:10,000. Anti-Laminin (Human) (RABBIT) Antibody was also assayed against 1.0 µg of Laminin in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. Specific conditions should be optimized by researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Laminin Antibody was produced by repeated immunizations with a mixture of synthetic peptides corresponding to regions of adherence on laminin.
• Purity: Anti-Laminin (RABBIT) Antibody has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Anti-Laminin (Human) (RABBIT) Antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Concentration: 1.04 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Laminin antibody, LAMA1 antibody, LAMB1 antibody, Laminin A chain antibody, Laminin alpha 1 antibody, Laminin alpha 1 chain antibody, Laminin B1 chain antibody, Laminin subunit beta 1 antibody

Immunohistochemistry of rabbit anti-Laminin antibody. Tissue: human placenta. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Laminin at 5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: Laminin as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of rabbit anti-Laminin antibody. Tissue: human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Laminin at 5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: Laminin as precipitated red signal with hematoxylin purple nuclear counterstain.

Quick Database Links

UniProt

P07942

RefSeq:AAA59486.1