Laminin alpha 1 Polyclonal Antibody

• Catalog Number: orb1417380
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Laminin alpha 1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Rat
• UniProt ID: P25391
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Laminin subunit alpha-1, Laminin A chain, Laminin-
• Research Area: Epigenetics
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Laminin alpha 1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Laminin alpha 1) Polyclonal Antibody, Unconjugated at 1: 500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.