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Item 1 of 9
Item 1 of 9
Lamin B1 Antibody, KO Validated
Catalog Number: orb1274472
| Catalog Number | orb1274472 |
|---|---|
| Category | Antibodies |
| Description | Lamin B1 Antibody, KO Validated |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human Lamin B1 (NP_005564.1). |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 70kDa |
| Target | LMNB1 |
| UniProt ID | P20700 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419, Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (orb1274472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts from normal (control) and Lamin B1 knockout (KO) HeLa cells, using Lamin B1 antibody (orb1274472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spinal cord using Lamin B1 antibody (orb1274472) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Lamin B1 antibody (orb1274472) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (orb1274472) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Lamin B1 Polyclonal Antibody (orb1274472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Lamin B1 Polyclonal Antibody (orb1274472) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
