Lambda Light Chain Antibody / Lambda Antibody

• Catalog Number: orb385740
• Category: Antibodies
• Description: This mAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HP6054
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Purified human IgG myeloma proteins were used as the immunogen for this Lambda Light Chain antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Lambda Light Chain antibody is available for research use only.
• UniProt ID: P01842, P01701
• Storage: Store the Lambda Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the Lambda Light Chain antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human tonsil using Lambda Light Chain antibody

Western blot analysis of human Intestinal lysate using Lambda Light Chain antibody