LDHA Antibody (Biotin)

SKU: orb344377

Description

Lactate Dehydrogenase antibody (Biotin)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, IP, WB
• Dilution Range: ELISA: 1:2,500 - 1:6,500, IP: 1:100, WB: 1:500 - 1:2,500
• Reactivity: Rabbit
• Application Notes:
  Anti-Lactate Dehydrogenase Biotin antibody has been tested by ELISA, Western blot, and immunofluorescence. This antibody is assayed against 1.0 µg of Lactate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

  Read more...

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Lactate Dehydrogenase [full length protein isolated from rabbit muscle]
• Target: LDHA
• Purity: Anti-lactate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. Cross reactivity against Lactate Dehydrogenase from other sources may occur but have not been specifically determined.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Lactate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M

Quick Database Links

Gene Symbol

LDHA

UniProt

P13491

RefSeq:126050