Lactate Dehydrogenase antibody (Biotin)

• Catalog Number: orb344377
• Category: Antibodies
• Description: Lactate Dehydrogenase antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IP, WB
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Lactate Dehydrogenase [full length protein isolated from rabbit muscle]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,500 - 1:6,500, IP: 1:100, WB: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: Anti-lactate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. Cross reactivity against Lactate Dehydrogenase from other sources may occur but have not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P13491
• NCBI: 126050
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Lactate Dehydrogenase Antibody biotin Co
• Note: For research use only
• Application notes: Anti-Lactate Dehydrogenase Biotin antibody has been tested by ELISA, Western blot, and immunofluorescence. This antibody is assayed against 1.0 µg of Lactate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Microscopy of Biotin conjugated Anti-Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.

Western Blot of Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.

Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 µg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.