LDHA Antibody (HRP)

SKU: orb344362

Description

Lactate Dehydrogenase antibody (Peroxidase)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution Range: ELISA: 1:3,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:6,000
• Reactivity: Rabbit
• Application Notes:
  Anti-Lactate Dehydrogenase peroxidase Antibody has been tested in ELISA, western blot, and immunofluorescence. This product is suitable for IP and IHC. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Lactate Dehydrogenase Antibody was produced by repeated immunizations with rabbit muscle Lactate Dehydrogenase full length protein.
• Target: LDHA
• Purity: Anti-Lactate Dehydrogenase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. Cross reactivity against Lactate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

goat anti-Lactate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M

Quick Database Links

Gene Symbol

LDHA

UniProt

P13491

RefSeq:126050