Lactate Dehydrogenase antibody (Peroxidase)

• Catalog Number: orb344362
• Category: Antibodies
• Description: Lactate Dehydrogenase antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Anti-Lactate Dehydrogenase Antibody was produced by repeated immunizations with rabbit muscle Lactate Dehydrogenase full length protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:3,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:6,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Lactate Dehydrogenase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. Cross reactivity against Lactate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• UniProt ID: P13491
• NCBI: 126050
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: goat anti-Lactate Dehydrogenase Antibody HRP Conju
• Note: For research use only
• Application notes: Anti-Lactate Dehydrogenase peroxidase Antibody has been tested in ELISA, western blot, and immunofluorescence. This product is suitable for IP and IHC. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using Lactate Dehydrogenase antibody (Peroxidase)

Western blot analysis of Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane using Lactate Dehydrogenase antibody (Peroxidase)